【摘 要】
：
目的:探讨A型核纤层蛋白前体( prelamin A)在细胞内堆积造成细胞早老的机理,筛选了prelamin A相互作用蛋白并研究其在早老细胞中的表达情况.方法:以prelamin A的C末端区域为
【机 构】
：
广东医学院衰老研究所,广东医学院生物化学与分子生物学研究所,广东省中医院检验科
论文部分内容阅读
目的:探讨A型核纤层蛋白前体( prelamin A)在细胞内堆积造成细胞早老的机理,筛选了prelamin A相互作用蛋白并研究其在早老细胞中的表达情况.方法:以prelamin A的C末端区域为诱饵蛋白,采用酵母双杂交方法从人骨骼肌cDNA文库中筛选prelamin A相互作用蛋白.构建了prelaminA识别因子(Narf)与绿色荧光蛋白融合表达载体pEGFP - Narf,与红色荧光蛋白- prelamin A融合表达质粒pDsRed - PLA共转染HEK293细胞,激光共聚焦显微观察共定位情况.Western blotting检测Narf在衰老表型HEK293PLA细胞的表达情况.结果:筛选得到包括Narf在内的7个候选相互作用蛋白.Narf与prelamin A能相互作用并共定位于核纤层,在prelamin A过表达的HEK293PLA细胞中Narf表达没有升高.结论:Narf在细胞内与prelamin A相互作用,且表达量不受后者影响.
其他文献
The innate toxicity of dichloroethenes(DCEs) and their tendency to be reduced to vinyl chloride(VC)(a known human carcinogen) is a cause for environmental conce
The low biodegradability of polyphenolic compounds typically found in olive processing indicated that biological treatment is not always successful in the treat
The reaction of triphenylphosphine with AgNO3 yielded an unusual nitrate anion bridging one-dimensional infinite chain [Ag(PPh3)NO3]∞ in which the silver(I) at
应用克隆的基因组序列作为同源臂,构建了小鼠β-酪蛋白基因定位敲人打靶载体.短臂长2.7kb,包括小鼠p-酪蛋白基因5端侧翼区、第1外显子、第1内含子、部分第2外显子.长臂包括小
Hydrophilic ZnS nanocrystals with narrow size distribution were synthesized via homogeneous precipitation using EDTA as stabilizer. The as-synthesized products
The utility of nickel/iron in the remediation of atrazine-contaminated water was investigated. The experimental results showed that nickel/iron had effective ca
This paper summarizes atmospheric aerosol concentrations of 5 stratospheric balloon soundings during the period from 1984 to 1994. Aerosol-rich layers in the tr
Program slicing can be effectively used to debug, test, analyze, understand and maintain objectoriented software. In this paper, a new slicing model is proposed
Some coupled 1D-2D hydrodynamic and water quality models depicting tidal water bodies with complex topography were presented. For the coupled models, finite ele
目的观察水通道蛋白1(AQP1)在小鼠口腔粘膜的表达与分布,为研究AQP1在口腔水代谢和感觉传递中的作用提供形态学依据。方法制备成年小鼠口腔各部粘膜石蜡切片,进行AQP1免疫组