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对经脱毒的优良苹果品种北海道９号、皇家嘎拉和长富２号进行了试管微繁技术的研究。结果表明：适合于３个品种试管苗增殖的培养基为ＭＳ＋６—ＢＡ０．５ｍｇ／Ｌ＋ＮＡＡ０．０５ｍｇ／Ｌ＋蔗糖３０ｇ／Ｌ，增殖率为２５～３，≥３ｃｍ的试管苗率约为５０％。通用的生根培养基为１／２ＭＳ＋ＩＡＡ０．５ｍｇ／Ｌ＋ＩＢＡ０．５ｍｇ／Ｌ＋ＮＡＡ０．１５ｍｇ／Ｌ＋蔗糖２５ｇ／Ｌ－肌醇，平均生根率保持在７５％左右。生根试管苗采用“一步法移栽”，平均成活率在７８％以上。
Micropropagation experiments were conducted on detoxified apple cultivars Hokkaido No. 9, Royal Gala and Chang Fu No.2. The results showed that the medium for multiplication of three plantlets was MS + 6-BA0.5mg / L + NAA0.05mg / L + sucrose 30g / L with the proliferation rate of2.5 ~ 3. . Common rooting medium was 1 / 2MS + IAA0.5mg / L + IBA0.5mg / L + NAA0.15mg / L + sucrose25g / L-inositol, the average rooting rate remained at about75%. The rooting test tube seedlings using “one-step transplanting”, the average survival rate of 78% or more.