利用石蜡切片的方法对萝卜雄性不育系RS-CMS2的小孢子母细胞发育过程进行了组织学观察。结果表明:萝卜雄性不育株在四分体时期开始出现败育现象;绒毡层细胞膨大,出现大的液泡;随后绒毡层细胞破裂,侵入药室,细胞彼此之间难以分清界限;小孢子粘连在一起,胞质进一步收缩,并开始出现降解。该研究为进一步揭示萝卜细胞质雄性不育的作用机制、提高育种效率有重要的参考价值。 The microspore blastocyst development of male sterile line RS-CMS2 of radish was observed by paraffin section. The results showed that the male sterile plants of radish began aborting in the tetrad stage. The tapetal cells became swollen with large vacuoles. Afterwards, the tapetal cells ruptured and invaded into the drug compartment, making it difficult to distinguish the cells from each other. The spores are stuck together, the cytoplasm shrinks further and begins to degrade. This study has important reference value for further revealing the mechanism of radish cytoplasmic male sterility and improving breeding efficiency.