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目的:探讨茯贞膏对贫血小鼠造血功能的影响。方法:选取128只BALB/c小鼠作为本次实验的研究对象,首先适应性喂养小鼠1周,然后运用物理辐射方法将小鼠进行环磷酰胺致贫血造模,造模工程结束后,将128只BALB/c小鼠按照随机数表法平均分为观察组与对照组,观察组64只小鼠,对照组64只小鼠。对两组小鼠采取不同的给药方式,给予观察组小鼠茯贞膏灌位给药,每日给药9 g/kg,9日为1个周期,连续给药1个周期;对照组给予生理盐水灌胃给药,服用剂量与观察组相同,给药周期为9 d。对两组小鼠治疗9 d后血象、骨髓有核细胞计数和脾脏指数变化情况等相关指标进行比较分析。结果:观察组小鼠治疗后RBC数值为(9.6±1.5)×10~(12)/L,对照组RBC数值仅为(7.9±1.8)×10~(12)/L,观察组RBC数值明显高于对照组数值,二者差异明显,具有统计学意义(P<0.05);观察组小鼠治疗后WBC数值为(19.7±1.3)×10~9/L,对照组WBC数值为(6.1±1.9)×10~(12)/L,对照组明显高于观察组,二者有明显差异,具有统计学意义(P<0.05);观察组小鼠治疗后HB数值为(199.5±5.6)g/L,明显高于对照组的(159.1±4.8)g/L,二者差异明显,具有统计学意义(P<0.05);观察组小鼠治疗后PLT数值为(488.2±11.4)×10~9/L,对照组小鼠治疗后PLT数值为(375.2±12.6)×10~9/L,观察组小鼠治疗后PLT数值明显高于对照组小鼠,有显著差异,具有统计学意义(P<0.05)。观察组患者治疗后骨髓有核细胞数(8.6±1.5)×10~9/L,对照组患者治疗后骨髓有核细胞数(17.9±1.8)×10~9/L,观察组明显高于对照组,二者差异明显,具有统计学意义(P<0.05)。观察组患者治疗后脾脏指数为(9.7±1.3)mg/g,对照组患者为(4.1±1.9)mg/g,观察组明显高于对照组,二者差异明显,具有统计学意义(P<0.05)。观察组小鼠各T细胞分泌细胞因子IFN-r分泌量为(17.9±1.8)pg/mL;TNF-a分泌量为(32.5±3.5)pg/mL;IL-4分泌量为(5.59±0.89)pg/mL;IL-6分泌量为(16.1±1.9)pg/mL。对照组小鼠IFN-r分泌量为(7.6±1.5)pg/mL;TNF-a分泌量为(21.5±2.5)pg/mL;IL-4分泌量为(4.48±0.23)pg/mL;IL-6分泌量为(7.7±1.3)pg/mL,观察组小鼠T细胞分泌的细胞因子各项指标均高于对照组小鼠,二者差异明显,具有统计学意义(P<0.05)。结论:茯贞膏能够显著改善贫血小鼠血象,对提高小鼠造血功能有积极影响,可试行二期临床。
Objective: To investigate the effect of Fushen cream on hematopoietic function in anemia mice. Methods: 128 BALB / c mice were selected as the object of this study. First, the mice were adaptively fed for 1 week. Then the mice were subjected to cyclophosphamide-induced anemia by physical radiation. After modeling, 128 BALB / c mice were equally divided into observation group and control group by random number table method, 64 mice in the observation group and 64 mice in the control group. The two groups of mice take different modes of administration, given the observation group mice Fuso cream irrigation, daily administration of 9 g / kg, 9 for 1 cycle, continuous administration of a cycle; control group Give saline administration, taking the same dose and observation group, the dosing period of 9 d. On the 9th day after treatment, the related indexes such as the blood picture, the number of bone marrow nucleated cells and the change of spleen index were compared and analyzed. Results: The RBC value in the observation group was (9.6 ± 1.5) × 10-12 / L after treatment, while the RBC value in the control group was (7.9 ± 1.8) × 10-12 / L. The RBC value in the observation group was significantly (19.7 ± 1.3) × 10 ~ 9 / L and WBC in the control group were (6.1 ± 1.9) × 10 ~ (12) / L, the control group was significantly higher than the observation group, there was a significant difference between the two groups (P <0.05); HB in the observation group was (199.5 ± 5.6) g / L, which was significantly higher than that of the control group (159.1 ± 4.8) g / L, with significant difference between the two groups (P <0.05). The PLT value in the observation group was (488.2 ± 11.4) 9 / L, the PLT value of the control group was (375.2 ± 12.6) × 10 ~ 9 / L after treatment, and the PLT value of the mice in the observation group was significantly higher than that of the control group (statistically significant P <0.05). The number of nucleated cells in the observation group was (8.6 ± 1.5) × 10 ~ 9 / L after treatment, and the number of bone marrow nucleated cells (17.9 ± 1.8) × 10 ~ 9 / L in the control group was significantly higher than that in the control group Group, the difference between the two was statistically significant (P <0.05). The spleen index of the observation group was (9.7 ± 1.3) mg / g after treatment, and (4.1 ± 1.9) mg / g in the control group, the observation group was significantly higher than that of the control group, with significant difference (P < 0.05). The secretion of cytokine IFN-γ secreted by each T cell in observation group was (17.9 ± 1.8) pg / mL, the level of TNF-α secretion was (32.5 ± 3.5) pg / mL and the level of IL-4 secretion was (5.59 ± 0.89 ) pg / mL; IL-6 secretion was (16.1 ± 1.9) pg / mL. The secretion of IFN-γ in the control group was (7.6 ± 1.5) pg / mL; the secretion of TNF-α was (21.5 ± 2.5) pg / mL; the secretion of IL-4 was 4.48 ± 0.23 pg / mL; -6 was (7.7 ± 1.3) pg / mL. The indexes of cytokines secreted by T cells in observation group were significantly higher than those in control group, with significant difference (P <0.05). Conclusion: Fushen cream can significantly improve the blood of anemic mice, to improve the hematopoietic function in mice have a positive effect, the pilot phase II clinical trials.