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目的探讨低剂量辐射对小鼠种植肿瘤生长的抑制作用及对红细胞免疫的兴奋效应。方法昆明种雄性小鼠右后肢腹股沟皮下接种S180肉瘤细胞,接种前6 h全身X线照射75 mGy,照射后5 d观察肿瘤发生率,隔日测量肿瘤体积。15 d后杀鼠,测量肿瘤质量,病理切片观察肿瘤坏死面积、肿瘤浸润性淋巴细胞。同时检测荷瘤小鼠红细胞免疫功能,2,3-DPG水平和超氧化物歧化酶(SOD)活性变化。结果与直接荷瘤组相比,低剂量照射组肿瘤生长明显减慢(P<0.05),肿瘤坏死面积增大。低剂量照射组红细胞免疫功能、2,3-DPG含量、SOD活性明显高于直接荷瘤组(P<0.05)。结论低剂量辐射可明显提高机体抗肿瘤的作用,提高红细胞免疫功能及SOD活性,增加2,3-DPG含量,可能具有肿瘤治疗的潜在临床意义。
Objective To investigate the inhibitory effect of low dose radiation on tumor growth of mice and the excitatory effect on erythrocyte immunity. Methods Kunming male mice were inoculated subcutaneously into the groin of the right hindlimb for S180 sarcoma cells. Whole body X-ray irradiation was performed at 75 mGy 6 h before inoculation. The tumor incidence was observed 5 d after irradiation and the tumor volume was measured on the next day. After 15 days, the mice were sacrificed and the tumor mass was measured. The tumor necrosis area and tumor infiltrating lymphocytes were observed by pathological sections. Meanwhile, the erythrocyte immune function, 2,3-DPG level and superoxide dismutase (SOD) activity in tumor-bearing mice were detected. Results Compared with the direct tumor-bearing group, the tumor growth of the low-dose irradiation group was significantly slowed down (P <0.05), and the tumor necrosis area increased. The erythrocyte immune function, 2,3-DPG content and SOD activity in low-dose irradiation group were significantly higher than those in direct tumor-bearing group (P <0.05). Conclusion Low-dose radiation can significantly improve the anti-tumor effect of the body, improve erythrocyte immune function and SOD activity, and increase the content of 2,3-DPG, which may have the potential clinical significance of tumor therapy.