论文部分内容阅读
背景:脑干孤束核是内脏痛信息向高位脑中枢传递的一个关键的中继站。形态学证据表明,大鼠孤束核中分布有大量的神经激肽B受体(neurokininBreceptors,NKR)。然而,孤束核中表达NKR的神经元是否参与内脏痛信息的传入与整合目前仍不清楚。目的:阐明孤束核中表达NKR的神经元在脑肠通路中传递内脏痛信息中的作用。设计:随机的对照实验性研究。地点和对象:12只SD大鼠购自第四军医大学实验动物中心。大鼠均为雄性,体质量220～250g,用随机数字表法分为实验组和对照组,每组各6只。干预:6只雄性大鼠吸入麻醉后,将2mL50g/L的乙酸经导管注入大鼠升结肠内,20s后再注入5mL生理盐水冲洗结肠。对照组大鼠则注射等体积的磷酸缓冲液(0.01mol/L)。24h后大鼠开胸经心脏灌流40g/L的甲醛。灌注毕后取出鼠脑修块,然后将含有孤束核的脑块在300g/L的蔗糖溶液中浸泡过夜,冰冻切片机切片。用抗FOS抗体和抗NKR抗体行免疫组化双重标记染色。免疫组化由第一作者完成,动物模型构建由第二作者完成,第三及第四作者负责免疫组化结果评估。主要观察指标:孤束核中NKR和FOS蛋白的共表达。结果:孤束核各个亚核中均有大量的NKR免疫阳性的神经元,但在内侧亚核和联合亚核中尤其浓密。阳性产物分布在细胞膜和树突上;胃肠道给予甲醛刺激后,表达FOS蛋?
Background: The solitary nucleus of the brain stem is a key relay for the transmission of visceral pain information to the upper brain center. Morphological evidence shows that a large number of neurokinin B receptors (NKRs) are distributed in the nucleus tractus solitarius in rats. However, whether or not neurons expressing NKR in the nucleus tractus solitarius are involved in the introduction and integration of visceral pain information remains unclear. OBJECTIVE: To elucidate the role of NKR-expressing neurons in the nucleus tractus solitarius in the transmission of visceral pain messages in the cerebro-intestinal tract. Design: Randomized controlled experimental study. Location and Subjects: Twelve SD rats were purchased from Experimental Animal Center of the Fourth Military Medical University. Rats were male, body weight 220 ~ 250g, using random number table divided into experimental group and control group, each group of 6. Intervention: 6 male rats inhaled anesthesia, 2mL50g / L of acetic acid into the ascending colon by catheter, 20s and then injected into the colon 5mL normal saline. Control rats were injected an equal volume of phosphate buffer (0.01mol / L). After 24h rat thoracotomy by cardiac perfusion 40g / L of formaldehyde. After perfusion, the rat brain was removed, and then the brain containing the nucleus of solitary tract was soaked in 300 g / L sucrose solution overnight. The frozen microtome was sliced. Immunohistochemical double staining with anti-FOS and anti-NKR antibodies. Immunohistochemistry was performed by the first author, animal models constructed by the second author, and third and fourth authors evaluated immunohistochemistry results. MAIN OUTCOME MEASURES: Co-expression of NKR and FOS proteins in nucleus tractus solitarius. Results: A large number of neurons positive for NKR immunoreactivity were found in all subnucleus of solitary tract nucleus, especially in medial subnucleus and united subnucleus. Positive products distributed on the cell membrane and dendrites; gastrointestinal tract to give formaldehyde after stimulation, the expression of FOS egg?