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目的根据吖啶红(AR)染料的荧光光谱特性,建立了60Coγ射线辐射剂量的检测新方法。方法用60Coγ射线辐射AR溶液,根据测得AR溶液的荧光强度(F),建立γ射线辐射剂量(D)与荧光强度之间的D-F定量关系。结果随着60Coγ射线辐射剂量的增强,吖啶红(AR)溶液的荧光强度F值下降。当λex=530 nm,λem=550 nm,AR溶液浓度c=1.0×10-5mol/L~1.0×10-4mol/L时,AR溶液浓度越大,定量测定的辐射剂量范围越宽(0 kGy~4 kGy),辐射剂量与荧光强度之间的线性定量关系越好(r=0.9996)。实验发现,与AR结构相似的共存物质、溶液酸度和辐射温度对检测结果影响很小。研究阐述了H2O2的影响机制。分别应用光谱、质谱等技术表征了60Coγ射线辐射导致AR荧光变化的机理。结论本方法大大减少了用物理方法测定辐射剂量时60Coγ射线对人体的损伤,并具有测定累积辐射剂量的优势。
OBJECTIVE To establish a new detection method of 60Coγ-ray dose based on the fluorescence spectrum of acridine red (AR) dye. Methods The AR solution was irradiated by 60Coγ ray. Based on the measured fluorescence intensity (F) of AR solution, the quantitative relationship between γ-ray dose (D) and fluorescence intensity was established. Results With the increase of 60Coγ-ray dose, F value of acridine red (AR) solution decreased. When the concentration of AR solution is higher when λex = 530 nm, λem = 550 nm and the concentration of AR solution is 1.0 × 10-5mol / L to 1.0 × 10-4mol / L, the radiation dose range of quantitative determination is wider (0 kGy ~ 4 kGy), the better the linear relationship between radiation dose and fluorescence intensity (r = 0.9996). The experiment found that, with the AR structure similar to coexisting substances, solution acidity and radiation temperature on the test results have little effect. The research described the mechanism of H2O2. The mechanism of AR fluorescence changes caused by 60Coγ-ray irradiation was characterized by spectroscopy and mass spectrometry, respectively. Conclusion This method greatly reduces the damage of human body caused by 60Coγ-ray when the radiation dose is measured by the physical method, and has the advantage of determining the cumulative radiation dose.