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以21份水稻(Oryza sativa)种质为材料,用1.5%Na Cl处理种子8天后测定发芽率。在苗期用不同浓度NaCl水培处理10天,测定叶片死亡率等指标和高亲和性K+转运基因(HKT)家族变异。在成株期选3份种质,用不同浓度NaCl盆栽处理,在开花期和籽粒蜡熟期测定植株可溶性糖和生物量等指标,以明确各种质不同生育期的耐盐差异和关键指标。结果表明,在NaCl胁迫下,种子发芽率受到显著影响。苗期盐胁迫后,各种质的平均叶片死亡率变幅最大。在被鉴定的8个耐盐种质中,HKT家族的7个基因除OsHKT2;4外均存在。在≤1 g·kg–1盐胁迫下植株可溶性糖含量表现出刺激增长效应。CG15R单株生物量与盐浓度呈正相关,且随盐浓度的增加而缓慢增长。在≤1 g·kg–1时,中花9号的生物量随盐浓度的增加而增加。水稻耐盐性具有明显的阶段发育特异性,且不同发育阶段的耐盐性之间无相关性。叶片死亡率与蜡熟期生物量可分别作为苗期和成株期耐盐鉴定的关键指标。CG15R可作为高耐盐种质进行深入分析和利用。
The germination rate of 21 Oryza sativa accessions treated with 1.5% NaCl was measured after 8 days. The seedlings were treated with different concentrations of NaCl for 10 days to determine leaf mortality and other high-affinity K + transport gene (HKT) family variations. Three germplasms were selected in the adult stage and treated with NaCl at different concentrations. The soluble sugar and biomass of the plants were measured during the flowering and wax ripening periods to determine the salt tolerance and key indicators . The results showed that seed germination rate was significantly affected by NaCl stress. Seedling salt stress, the average variety of leaves the largest change in the rate of leaf mortality. Among the eight salt-tolerant germplasm identified, seven genes of HKT family except OsHKT2; 4 were present. Under the condition of ≤1 g · kg-1 salt stress, the soluble sugar content of plant showed a stimulative growth effect. The biomass of CG15R was positively correlated with salt concentration and slowly increased with salt concentration. At ≤1 g · kg-1, the biomass of Zhonghua 9 increased with the increase of salt concentration. Salt tolerance of rice has obvious stage developmental specificity, and there is no correlation between salt tolerance at different developmental stages. Leaf mortality and waxy biomass could be used as key indicators of salt tolerance in seedling and adult plants, respectively. CG15R can be used as a high salt tolerant germplasm for further analysis and utilization.