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目的研究Foxp3及相关基因在重症肌无力(MG)胸腺组织中的表达,探讨其在MG发病中的作用及意义。方法采用免疫组织化学、Western blot以及RT-PCR技术,检测MG患者及正常对照胸腺组织中Foxp3、Smad3、Smad7、TGF-β、IFN-γ的表达差异。结果MG胸腺Foxp3蛋白及mRNA表达均显著低于正常,MGFAⅡ、Ⅲ型(全身型)患者胸腺Foxp3表达显著低于Ⅰ型(眼肌型);而Ⅲ型(中度全身型)则显著低于Ⅱ型(轻度全身型)(P<0.05);MG胸腺内TGF-βmRNA水平及其信使Smad3/Smad7比值显著降低,与Foxp3表达呈显著正相关(P<0.01,r=0.871)。结论Foxp3在MG发病中可能起着重要作用,TGF-β表达异常可能系MG胸腺Foxp3下调的原因之一。
Objective To investigate the expression of Foxp3 and its related genes in myasthenia gravis (MG) thymus and to explore its role in the pathogenesis of MG. Methods The expressions of Foxp3, Smad3, Smad7, TGF-β and IFN-γ in MG patients and normal control thymus tissues were detected by immunohistochemistry, Western blot and RT-PCR. Results The expression of Foxp3 protein and mRNA in MG thymus were significantly lower than those in normal group. The expression of Foxp3 in thymus of patients with MGFA Ⅱ and Ⅲ type was significantly lower than that of type Ⅰ (ocular muscle type), while the type Ⅲ (moderate whole body) (P <0.05). The level of TGF-βmRNA and the ratio of messenger Smad3 / Smad7 in MG thymus were significantly decreased, which was positively correlated with Foxp3 expression (P <0.01, r = 0.871). Conclusion Foxp3 may play an important role in the pathogenesis of MG. Abnormal expression of TGF-β may be one of the reasons for the down-regulation of Foxp3 in MG thymus.