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目的:探讨体外模拟正畸牙移动过程中机械压力对人牙周膜干细胞(HPDLSC)凋亡相关蛋白表达的影响。方法:将体外培养的HPDLSC分别在自行研发的加压力装置中进行100、200 Kpa加压1、6和12 h后,检测和比较其凋亡相关蛋白caspase-3和caspase-8的表达;流式细胞仪检测其凋亡状态。结果:100 Kpa加压1 h的HPDLSCs中caspase-3和caspase-8的蛋白表达及其凋亡水平均显著高于对照组(P<0.05),而加力12 h的HPDLSCs中caspase-3和caspase-8的蛋白表达和凋亡水平与对照组比较无统计学差异(P>0.05)。200 Kpa加力1 h的HPDLSCs中caspase-3和caspase-8的蛋白表达和凋亡水平均显著低于对照组(P<0.05),而加力12 h的HPDLSCs中caspase-3和caspase-8的蛋白表达和凋亡水平与对照组比较无统计学差异(P>0.05)。结论:在体外模拟正畸牙移动过程中,不同强度静态压力作用不同时间对人牙周膜干细胞的凋亡作用不同,100 Kpa静态加力加压1 h可更有效地促进人牙周膜干细胞的凋亡,而200 Kpa静态加力加压1 h可抑制人牙周膜干细胞的凋亡。
AIM: To investigate the effect of mechanical stress on the expression of apoptosis-related proteins in human periodontal ligament stem cells (HPDLSC) during orthodontic tooth movement in vitro. Methods: HPDLSC cultured in vitro were respectively tested for the expression of caspase-3 and caspase-8 at 1,600 and 12 h after pressurization at 100 and 200 Kpa respectively. Flow Cytometry was used to detect the apoptotic state. Results: The protein expression and apoptosis of caspase-3 and caspase-8 in HPDLSCs treated with 100 Kpa for 1 h were significantly higher than those in the control group (P <0.05), while the caspase-3 and There was no significant difference in the protein expression and apoptosis of caspase-8 compared with the control group (P> 0.05). The protein expression and apoptosis of caspase-3 and caspase-8 in HPDLSCs treated with 200 Kpa for 1 h were significantly lower than those in the control group (P <0.05), while caspase-3 and caspase-8 There was no significant difference in the protein expression and apoptosis between the two groups (P> 0.05). CONCLUSION: The apoptosis of human periodontal ligament stem cells is affected by the static pressure at different times during the simulated orthodontic tooth movement in vitro. The static pressure of 100 Kpa for 1 h can effectively promote the proliferation of human periodontal ligament stem cells The apoptosis of human periodontal ligament stem cells was inhibited by static pressure and pressure of 200 Kpa for 1 h.