目的测定唐古特大黄中2种鞣质类成分没食子酸和儿茶素的含量。方法用高效液相色谱法,流动相为甲醇-0.1%磷酸水溶液,采用梯度洗脱,检测波长为280nm,流速为1.0mL·min?1,柱温为室温,按外标法定量。结果没食子酸的平均加样回收率为98.6%,RSD为1.28%;儿茶素的平均加样回收率为98.8%,RSD为1.37%。结论所建立的方法可准确、快速有效地测定唐古特大黄药材中鞣质类物质的含量。 Objective To determine the content of gallic acid and catechin in two kinds of tannins in rhubarb of Tanggut. Methods The mobile phase consisted of methanol - 0.1% phosphoric acid aqueous solution with gradient elution at a detection wavelength of 280 nm and a flow rate of 1.0 mL · min -1 at room temperature. The method was characterized by external standard method. Results The average recoveries of gallic acid were 98.6% with RSD of 1.28%. The average recoveries of catechins were 98.8% with RSD of 1.37%. Conclusion The established method can accurately, quickly and effectively determine the contents of tannins in the rhizoma of Tanggut rhubarb.