近年来,消化道的内分泌细胞不断受到重视。这些细胞的特点是细胞基部的胞质内含有各种大小不同的颗粒,这些颗粒对银盐具有一定的亲和力。因此显示颗粒大小、形态、分布、密度等是鉴定细胞的重要方面,在以往的研究中,人们把颗粒列Grimelius银法有无反应,或者反应强弱作为鉴定颗粒的重要方法之一。比如,分布在胃底腺、幽门腺及十二指肠前段肠腺内的D细胞对Grimelius银法无反应,而人胚、猴、猫、狗等胃底腺深部的A细胞中颗粒芯周围则对Grimelius银法反应阳性。这些都说明Grimlius银法,在鉴定胃肠内分泌细胞颗粒中与其他银法有不同的作用,所以如何使Grimelius银法的镀染效果发挥到更良好的 In recent years, gastrointestinal endocrine cells continue to receive attention. These cells are characterized by the cytoplasm at the base of the cell containing a variety of different sized particles that have some affinity for the silver salt. Therefore, particle size, morphology, distribution, density and so on are important aspects of cell identification. In the previous studies, one of the important methods for identifying particles was whether Grimelius silver method was used or not, or the reaction strength was used. For example, D cells distributed in the fundic gland, the pyloric gland and the duodenum in the anterior gland of the duodenum do not respond to the Grimelius silver method, whereas the A-cells in the A cells deep in the fundic gland of human embryos, monkeys, cats, dogs and the like Grimelius silver law positive reaction. All of this shows that the Grimlius silver method has a different role in the identification of gastrointestinal endocrine cells than other silver methods, so how to make the Grimelius silver plating method to a better effect