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Aim:To examine the protective effect of propofol in renal ischemia/reperfusion(I/R)injury and the role of heme oxygenase-1(HO-1)in this process.Methods:Sprague-Dawley rats were randomly divided into 3 groups:(i)sham-operatedgroup;(ii)I/R group;and(iii)propofol group.Bilateral renal warm ischemia for 45min was performed.After 2,6,and 24 h reperfusion,blood samples and kidneyswere collected for assessment of renal injury,and HO-1 expressions were ana-lyzed by immunohistochemical analysis,RT-PCR and Western blotting.Results:Blood urea nitrogen and serum creatinine levels in the propofol group were sig-nificantly lower than that in the I/R group at 24 h after reperfusion.The meanhistological score by Paller’s standard showed that propofol significantly attenu-ated renal I/R injury after 6 h reperfusion.Propofol increased HO-1 mRNA andprotein levels 2 h after reperfusion,whereas HO-1 expressions were present atexceedingly low levels in the I/R group and the sham-operated group at same timepoint.Propofol also markedly increased HO-1 mRNA and protein levels than I/Rat 6 and 24 h after reperfusion.Conclusion:These results suggest that propofolmitigates renal I/R injury in rats.This protection may be partly through the induc-tion of the HO-1 expression.
Aim: To examine the protective effect of propofol in renal ischemia / reperfusion (I / R) injury and the role of heme oxygenase-1 (HO-1) in this process. Methods: Sprague-Dawley rats were randomly divided into 3 groups: (ii) I / R group; and (iii) propofol group.Bilateral renal warm ischemia for 45 min was performed. After 2, 6 and 24 h reperfusion, blood samples and kidneys were collected for assessment of renal injury, and HO-1 expressions were ana-lyzed by immunohistochemical analysis, RT-PCR and Western blotting. Results: Blood urea nitrogen and serum creatinine levels in the propofol group were sig-nificantly lower than that in the I / R group at 24 h after reperfusion. The mean histological score by Paller’s standard showed that propofol significantly attenuated a-renal I / R injury after 6 h reperfusion .ropofol increased HO-1 mRNA and protein levels 2 h after reperfusion, even HO-1 expressions were present atexceedingly low levels in the I / R group and the sham-operated group at same timep These results suggest that propofolmitigates renal I / R injury in rats. This protection may be partly through the induc tion of the HO-1 expression.