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目的 :探讨大肠癌细胞转移能力与肿瘤相关因素的关系。方法 :用结肠癌 HT- 2 9亚系 HT- 2 9c和 HT- 2 9d细胞在裸大鼠体内建立转移模型 ,比较其与母系的转移能力。用 ELISA法测定 3个大肠癌细胞系的尿激酶型纤溶酶原激活因子 (u PA)及纤溶酶原抑制剂 - 1(PAI- 1)含量 ;用免疫组化技术检测癌胚抗原和 3-磷酸肌醇激酶(PI3- Kinase)在体内外的表达 ;用流式细胞仪测定癌胚抗原表达。结果 :在裸鼠体内 HT- 2 9d细胞的肝转移率明显高于母系 HT- 2 9细胞 ,转移累及的脏器增多。在体外培养中 ,HT- 2 9d细胞的 u PA及 PAI- 1含量明显高于 HT- 2 9和 Wi Dr细胞。在裸鼠体内 ,HT- 2 9d细胞的 PI3- Kinase表达明显高于 HT- 2 9和 Wi Dr细胞。结论 :经裸鼠体内筛选的大肠癌 HT- 2 9细胞亚系表现出增强的肿瘤转移能力。UPA、PAI- 1的含量及 PI3- Kinase的表达与肿瘤转移能力有关。
Objective: To investigate the relationship between tumor metastasis and tumor related factors in colorectal cancer cells. Methods: The metastatic models were established in HT-2 9 colon cancer HT-2 9c and HT-2 9d cells in nude rats, and their metastatic potential was compared with that of their matrices. Urokinase-type plasminogen activator (u PA) and plasminogen inhibitor-1 (PAI-1) were measured by ELISA in 3 human colorectal cancer cell lines. Immunohistochemistry was used to detect the expression of carcinoembryonic antigen 3-phosphoinositide kinase (PI3-Kinase) expression in vitro and in vivo; flow cytometry was used to determine the expression of carcinoembryonic antigen. Results: The rate of hepatic metastasis of HT-2 9d cells in nude mice was significantly higher than that of the parental HT-29 cells, and the number of metastatic organs increased. In vitro culture, u PA and PAI-1 levels in HT-29d cells were significantly higher than HT-29 and Wi Dr cells. In nude mice, PI3-Kinase expression in HT-29d cells was significantly higher than HT-29 and Wi Dr cells. CONCLUSIONS: Colorectal cancer HT-2 9 cell sublineages screened in nude mice exhibit enhanced tumor metastasis. The contents of UPA and PAI-1 and the expression of PI3-Kinase are related to tumor metastasis.