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应用V_(79)地鼠肺纤维细胞体外微核率的测定方法,比较不同地区及厂牌的五种民用鱼露的致染色体损伤作用,结果在62.5μl/ml的浓度,作用24hr,以南澳散装鱼露FS_1致微核率为最高。 我们试图用维胺酸(RⅡ)降低其微核率,结果鱼露浓度较高(60～70μl/ml)时,维胺酸对微核出现只有轻度的抑制作用,而鱼露浓度在48～56μl/ml以下,RⅡ对微核率抑制作用很强(降低一倍以上)。 我们还用小分子干扰素诱导剂Tilorone进行实验,发现其作用程度相似,在适当剂量下(10—20μg/ml),对染色体损伤有保护作用,可使微核率降低。两种抑制因子比较,尤以RⅡ作用稳定,对细胞生长亦无损害。
Using V_(79) murmur pulmonary fibroblasts in vitro micronucleus rate method to compare the chromosomal damage induced by five kinds of civilian fish sauce in different regions and brands, the result was at a concentration of 62.5μl/ml for 24hrs to Nanao The micronucleus rate of bulk fish sauce FS 1 was the highest. We tried to reduce the micronucleus rate with ribonucleotide (RII). As a result, when the concentration of fish sauce was high (60-70μl/ml), vitamic acid only slightly inhibited the appearance of micronuclei, and the fish sauce concentration was 48. Below -56 μl/ml, RII has a very strong inhibitory effect on micronucleus rates (more than a factor of two). We also used small-molecule interferon-inducing agent Tilorone to perform an experiment and found that the effect was similar. Under the appropriate dose (10-20 μg/ml), it had a protective effect on chromosome damage and reduced the micronucleus rate. Compared with the two inhibitory factors, the effect of RII is stable, and there is no damage to cell growth.