用氚化胸腺嘧啶核苷(~3H—TdR)掺入法测定有丝分裂原PHA刺激人外周血淋巴细胞转化反应。观察到在PHA浓度为50μg/ml时,5×10~5单个核细胞/ml的转化刺激指数最高。当细胞数继续增加,其刺激指数增加不明显。用自然沉降与密度梯度离心两种方法分离的淋巴细胞对PHA 刺激的反应不同。在无PHA刺激的情况下,自然沉降淋巴细胞的自然转化较密度梯度离心法分离者为高。 The mitogen-activated thymidine (~3H-TdR) incorporation assay was used to determine the mitogen-induced PHA stimulation of human peripheral blood lymphocyte transformation. It was observed that at a PHA concentration of 50 [mu]g/ml, the conversion stimulation index of 5x10~5 mononuclear cells/ml was the highest. As the number of cells continues to increase, its stimulation index does not increase significantly. Lymphocytes isolated by natural sedimentation and density gradient centrifugation have different responses to PHA stimulation. In the absence of PHA stimulation, the natural conversion of naturally sedimented lymphocytes was higher than that of density gradient centrifugation.