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在HCl-NaAc酸性缓冲介质中,曙红Y(EY)与马来酸罗格列酮(ROM)反应形成1∶1的离子缔合物,不仅引起吸收光谱(UV)的变化和EY的荧光猝灭(FS),更能导致共振瑞利散射(RRS)的显著增强,并产生新的RRS光谱。以试剂为参比,吸收光谱的最大褪色波长为510nm;荧光猝灭的激发波长λex和发射波长λem分别为521和544nm;最大共振散射波长λRRS为307nm,并在532nm处形成较强的散射峰。各方法的线性范围分别为4.98×10-7～7.5×10-6 mol/L(UV)、8.34×10-8～1.79×10-5 mol/L(FS)和4.66×10-9～1.25×10-5 mol/L(RRS),检出限分别为1.49×10-7 mol/L(UV)、2.50×10-8 mol/L(FS)和1.40×10-9 mol/L(RRS)。文中研究了吸收、荧光和共振散射的光谱特征、适宜的反应条件及影响因素,并对荧光猝灭机制及RRS的增强进行了讨论,据此建立了灵敏、简便、快速测定抗糖尿病药物马来酸罗格列酮的新方法。
Eosin Y (EY) reacts with rosiglitazone maleate (ROM) in a HCl-NaAc acid buffer medium to form a 1: 1 ion association that causes not only changes in the absorption spectrum (UV) and fluorescence of EY Quenching (FS) can lead to a significant increase of Resonance Rayleigh Scattering (RRS) and generate a new RRS spectrum. Reagent as a reference, the absorption spectrum of the maximum fading wavelength of 510nm; fluorescence quenching excitation wavelength λex and emission wavelength λem were 521 and 544nm; the maximum resonance wavelength λRRS 307nm, and at 532nm to form a strong scattering peak . The linear range of each method was 4.98 × 10-7 ~ 7.5 × 10-6 mol / L (UV), 8.34 × 10-8 ~ 1.79 × 10-5 mol / L (FS) and 4.66 × 10-9 ~ 1.25 The detection limits were 1.49 × 10-7 mol / L (UV), 2.50 × 10-8 mol / L (FS) and 1.40 × 10-9 mol / L (RRS) ). In this paper, the spectral characteristics of absorption, fluorescence and resonance scattering, the appropriate reaction conditions and influencing factors were studied. The mechanism of fluorescence quenching and the enhancement of RRS were also discussed. A sensitive, simple and rapid method for determining the anti-diabetic drug Malay A new method of acid rosiglitazone.