以夏氏疟原虫(Plasmodium chabaudi chabaudi)AS株配子体免疫小鼠后,制备了两种单克隆抗体,McAb5E(5E)及McAb5F(5F);5E既有阻断疟疾传播的活性又有对抗红细胞内期无性体的保护作用。通过间接免疫荧光试验,5E可与各无性阶段、配子体及小配子起反应,而5F则仅与环状体、配子体及小配子起作用。这种抗无性体及阻断传播的活性是由于无性体与配子体有共同的表位,还是由于交叉反应所致,尚待进一步研究。 Two kinds of monoclonal antibodies, McAb5E (5E) and McAb5F (5F), were prepared after mice were immunized with Plasmodium chabaudi AS strain. The 5E had both the activity of blocking malaria transmission and the ability of antagonizing erythrocyte Period of asexual protection. By indirect immunofluorescence assay, 5E reacts with asexual stages, gametocytes and gametes, while 5F functions only with the annulus, gametocytes and gametes. Whether such antisera and the activity of blocking the transmission are due to the common epitope of the vegetative and gametophyte or to the cross-reaction remains to be further studied.