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利用补骨脂素(Psoralen)在可见光照射下可与DNA双链结合的性质,Sheldon提出在DNA探针的末端连结一段双链DNA,用连有生物素的补骨脂素与其光反应则可在DNA探针的末端标记生物素。据报道其检测灵敏度可达1×10~4拷贝。我们在研究补骨脂素与双涟DNA光反应时发现,补骨脂素不仅可与双链DNA交联而且也可与单链DNA结合,与补骨脂
Taking advantage of the property that Psoralen binds to DNA double-stranded under visible light irradiation, Sheldon proposed to attach a double-stranded DNA at the end of the DNA probe and react with the light with psoralen with biotin Biotin is labeled at the end of the DNA probe. It is reported that the detection sensitivity of up to 1 × 10 ~ 4 copies. We study the psoralen and double ripple DNA light reaction found that psoralen not only with double-stranded DNA cross-linked but also with single-stranded DNA binding, and psoralen