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目的:初步探讨卵泡液中双酚A(BPA)浓度与卵巢储备功能下降(DOR)的相关性。方法:于取卵日收集行体外受精/卵胞质内单精子显微注射(IVF/ICSI)的DOR患者和非DOR患者的卵泡液,酶联免疫吸附(ELISA)技术测定54例DOR患者及67例非DOR患者卵泡液中BPA浓度及激素水平,采用SPSS 17.0软件进行统计学分析。结果:DOR组较非DOR组卵泡液中BPA浓度显著升高[(234.048±81.736)ng/L vs(193.300±67.225)ng/L,P<0.01];DOR组较非DOR组卵泡液中抗苗勒氏管激素(AMH)[(555.689±74.224)pg/ml vs(587.178±77.731)pg/ml,P<0.05]及雌二醇(E2)[(209.720±31.556)pg/ml vs(221.845±32.632)pg/ml,P<0.05)水平低;且卵泡液中BPA与AMH及E2水平有弱负相关性(rBPA&AMH=-0.312,P<0.05;rBPA&E2=-0.290,P<0.05)。结论:DOR患者卵泡液中BPA浓度显著偏高,卵泡液BPA浓度与卵泡液AMH和E2浓度呈弱负相关性;BPA可能通过调节卵泡液中激素水平来影响卵巢储备功能。
Objective: To investigate the relationship between the concentration of BPA and the decline of ovarian reserve (DOR) in follicular fluid. Methods: Follicular fluid of DOR patients and non-DOR patients who underwent in vitro fertilization / intracytoplasmic sperm injection (IVF / ICSI) were collected on the day of ovulation. The serum levels of follicular fluid in 54 patients with DOR and 67 patients with DOR were measured by enzyme linked immunosorbent assay (ELISA) Cases of non-DOR follicular fluid in patients with BPA concentrations and hormone levels, using SPSS 17.0 software for statistical analysis. Results: Compared with non-DOR group, the level of BPA in follicular fluid of DOR group was significantly higher than that of non-DOR group (234.048 ± 81.736 ng / L vs 193.300 ± 67.225 ng / L, P <0.01) (555.689 ± 74.224) pg / ml vs (587.178 ± 77.731) pg / ml, P <0.05] and estradiol [(209.720 ± 31.556) pg / ml vs ± 32.632) pg / ml, P <0.05). There was a weak negative correlation between BPA and AMH and E2 levels in follicular fluid (rBPA & AMH = -0.312, P <0.05; rBPA & E2 = -0.290, P <0.05). CONCLUSION: The concentration of BPA in follicular fluid of DOR patients is significantly higher. The concentration of BPA in follicular fluid is weakly negatively correlated with the concentration of AMH and E2 in follicular fluid. BPA may affect ovarian reserve by regulating hormone levels in follicular fluid.