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为研究浓缩铀对新生大鼠脑发育的影响 ,给新生大鼠侧脑室内注射不同剂量的浓缩铀 2 μl (分别含2 3 5U 0、1、5和 10 μg)后 ,观察其对早期体格生长、行为发育的影响。以放射自显影示踪术观察脑内放射性核素行径定位 ,放射免疫技术检测超氧化物歧化酶 (SOD)、内皮素 (ET)的含量变化。结果显示 ,放射性核素行径主要定位于细胞核 ,在细胞浆和细胞间隙中亦有呈现 ;新生大鼠出现生长延迟及神经行为异常 ;随着浓缩铀剂量的增加 ,大鼠的小脑、皮质、海马、间脑中SOD、ET的含量有变化 ,小剂量组SOD和ET增升 ,而大剂量组却呈明显的抑制。结果提示 ,从生物化学角度表明 ,在新生大鼠中α辐射体浓缩铀对发育脑的损伤具有神经细胞的敏感性、易脆性和代偿性特征。
To study the effect of enriched uranium on brain development in neonatal rats, neonatal rats were injected intracerebroventricularly with different doses of 2 uranium enriched uranium (containing 235 U, 1, 5 and 10 ug respectively) Growth, the impact of behavioral development. The radionuclide localization was observed by autoradiography and the content of superoxide dismutase (SOD) and endothelin (ET) was detected by radioimmunoassay. The results showed that the radionuclide pathways were mainly located in the nucleus and were also found in the cytoplasm and intercellular space. The growth retardation and neurobehavioral abnormalities of neonatal rats appeared. With the increase of the dosage of uranium, the cerebellum, cortex and hippocampus , The contents of SOD and ET in the diencephalon changed, the SOD and ET in the low dose group increased, but the high dose group showed obvious inhibition. The results suggest that from the biochemical point of view, in neonatal rats enriched uranium uranium on the development of brain injury with nerve cell sensitivity, brittleness and compensatory characteristics.