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利用砂培体系继代培养不同病区甜菜多粘菌(Polymyxabetae),经酶联检测,分离得到2个带毒率高的分离株N,HR12。应用甜菜坏死黄脉病毒(BNYVV)抗血清和免疫金标记技术分析了甜菜根中P.betae不同发育阶段与病毒的关系。在初生原质体、游动孢子囊以及未成熟的游动孢子中观察到被金颗粒标记的病毒粒子,在休眠孢子外围也观察到金标记的病毒粒子,但在休眠孢子内未直接观察到病毒粒子,只是在其内壁及液泡中常见有标记上的金颗粒。
Using sand culture system, polymyxabetae was subcultured in different wards. Two isolates with high virulence rates, HR12 and HR12, were isolated and identified by enzyme-linked immunosorbent assay (ELISA). The beet root was analyzed by using anti-BNYVV antiserum and immunogold labeling technique. betae different stages of development and the relationship between the virus. Virions tagged with gold particles were observed in primordial protoplasts, zoospores, and immature zoospores, and gold-labeled virions were also observed in the periphery of resting spores, but not directly observed in resting spores Virus particles, but in its inner walls and vacuoles are commonly marked with gold particles.