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目的:探讨动脉粥样硬化(AS)兔血清和粥样硬化斑块中纤溶酶原激活物抑制物-1(PAI-1)的表达以及与血脂的相关性。方法:雄性大耳白兔随机分成正常饮食组和高脂饮食组,每组8只,饲养16周。2组动物均于饲养0、8、16周采耳缘静脉血,检测血清中胆固醇(TC)、低密度脂蛋白(LDL)以及PAI-1的水平;16周后处死动物,应用免疫组化方法检测PAI-1在主AS斑块中的表达。结果:正常饮食组0、8、16周血清中TC、LDL和PAI-1水平差异无统计学意义(均P>0·05);高脂饮食组8周后血清中TC、LDL和PAI-1水平较0周显著增加(均P<0·01),16周较8周增加更为明显(均P<0·01);免疫组化结果显示高脂饮食组主动脉壁PAI-1的表达明显高于对照组(P<0·01)。结论:AS的发生伴有血清和粥样斑块中PAI-1表达增加,表达量与血清TC、LDL呈正相关。
Objective: To investigate the expression of plasminogen activator inhibitor-1 (PAI-1) in serum and atherosclerotic plaque of atherosclerosis (AS) rabbits and its relationship with serum lipids. Methods: Male white rabbits were randomly divided into normal diet group and high fat diet group, with 8 rabbits in each group and fed for 16 weeks. The rabbits in both groups were anesthetized with 0, 8 and 16 weeks old rabbits for the detection of the level of cholesterol (TC), low density lipoprotein (LDL) and PAI-1. After 16 weeks, Methods The expression of PAI-1 in the main AS plaques was detected. Results: The levels of TC, LDL and PAI-1 in serum of 0, 8 and 16 weeks in normal diet group had no significant difference (all P> 0.05) 1 levels increased significantly (all P <0.01) in 16 weeks compared with those in the 8th week (all P <0.01). Immunohistochemistry showed that PAI-1 in the aortic wall of high- The expression was significantly higher than the control group (P <0.01). Conclusions: The expression of PAI-1 in serum and atherosclerotic plaques is associated with the occurrence of AS. The expression of PAI-1 is positively correlated with serum TC and LDL.