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用皮质素和皮质固酮注射对照的,饥饿的以及饥饿并切除肾上腺的大鼠,经过4,8,12,24和96小时,在这些人鼠身上测定乳酸脱氢酶(Ⅰ),磷酸甘油醛脱氨酶(Ⅱ),α-磷酸甘油脱氢酶(Ⅲ),二磷酸果糖醛缩酶(Ⅳ),苹果酸脱氢酶(V),6-磷酸葡萄糖酸脱氢酶(Ⅵ)以及谷氨酸-草酰乙酸氨基移换酶(Ⅶ),谷氨酸-丙酮酸氨基移换酶(Ⅷ)和色氨酸吡咯酶(Ⅸ)的活性。除此之外,还测定了肝脏重量和肝中蛋白质和糖元含量。发现了,固醇类化合物抑制(Ⅰ)的活性和提高(Ⅱ),(Ⅸ),(Ⅷ),(Ⅵ),(Ⅶ)的活性。饥饿并切除肾上腺的动物仅在注射对固醇类化合物之后而发生肝中糖元含量上升的那个时期,才可观察到(Ⅸ),(Ⅱ),(Ⅲ)和(Ⅵ)的活性作用。根据这些材料,作者认为,仅此四种酶才被皮质素直接诱导生成,而(Ⅴ),(Ⅲ),(Ⅶ),(Ⅳ)活性的改变可看成是续发的。
Control, starved, and starved adrenal rats were injected with corticosterone and corticosterone, and lactate dehydrogenase (I), phosphoglycerol Aldehyde deaminase (II), alpha-glycerophosphate dehydrogenase (III), fructose bisphosphatase (IV), malate dehydrogenase (V), 6- phosphogluconate dehydrogenase (VI) Glutamate-oxaloacetate aminotransferase (VII), glutamate-pyruvate aminotransferase (VIII) and tryptophan pyrrolidine (IX). In addition, the liver weight and liver protein and glycogen content were also determined. It has been found that steroid compounds inhibit the activity of (I) and enhance the activities of (II), (IX), (VIII), (VI) and (VII). (IX), (II), (III) and (VI) were observed only during starvation and adrenalectomy animals only during the period when hepatic glycogen content increased following the injection of steroids. According to these materials, the authors believe that only the four enzymes were directly induced by cortin, and the changes of (V), (III), (VII) and (IV) activity can be considered as renewal.