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为探讨人巨细胞病毒（ＨｕｍａｎＣｙｔｏｍｅｇａｌｏｖｉｒｕｓ，ＨＣＭＷ）ＡＤ１６９毒株体外感染兔胚肺细胞（ＲａｂｂｉｔＥｍｂｒｙｏＬｕｎｇｃｅｌ，ＲＥＬ）的敏感性，建立ＨＣＭＶＡＤ１６９毒株感染模型，将ＨＣＭＶＡＤ１６９毒株定量接种于ＲＥＬ，观察细胞病理变化；用单克隆抗体－免疫组化方法检测细胞内ＨＣＭＶ抗原；电镜检测细胞内病毒颗粒，用ＥＬＩＳＡ方法检测经ＲＥＬ增殖病毒培养物ＨＣＭＶ抗原滴度，用人胚肺细胞（ＨｕｍａｎＥｍｂｒｙｏＬｕｎｇｃｅｌ，ＨＥＬ）滴定病毒毒力变化。结果表明，接种病毒后，ＲＥＬ于４８ｈ出现细胞肿胀，胞浆内出现折光性颗粒，逐渐变圆、脱落，其病变特征与该病毒在ＨＥＬ增殖出现的病变一致；用免疫组化法在胞浆及其核内查到ＨＣＭＶ抗原；用透射电镜观察，在细胞核内查到大量病毒颗粒；用ＥＬＩＳＡ方法在ＲＥＬ病毒培养物中查到ＨＣＭＶ抗原，抗原滴度与ＨＥＬ增殖的病毒一致；与ＨＥＬ增殖物比较，经ＲＥＬ增殖的病毒培养物毒力降低１００ＴＣＩＤ５０。表明ＲＥＬ是ＨＣＭＶＡＤ１６９毒株的敏感宿主细胞。
In order to investigate the sensitivity of HC16 strain in vitro to Rabbit Embryo Lung cells (REL) in order to explore the infection model of HCMVAD169 strain, HCMVAD169 strain was inoculated into REL quantitatively to observe the cell pathological changes. The intracellular HCMV antigen was detected by monoclonal antibody immunohistochemistry, the intracellular virus particles were detected by electron microscopy, the titer of HCMV antigen was detected by ELISA assay, the titer of virus was determined by human Embryo Lungcel (HEL) Variety. The results showed that, after inoculation of virus, REL appeared cell swelling in 48h, there were refraction particles in the cytoplasm, gradually rounded off, and its pathological features were consistent with the virus in HEL proliferation of lesions; immunohistochemistry in the cytoplasm And found in the nucleus HCMV antigen; observed by transmission electron microscopy, a large number of virus particles were found in the nucleus; detected by ELISA ELISA HCMV antigen in the culture of the virus, antigen titer and HEL proliferation of the virus; In comparison, virulence of virus cultures propagated via REL was reduced by 100 TCID50. REL is a sensitive host cell for the HCMVAD169 strain.