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目的探讨纳米硫化铅对雄性大鼠精子生成的影响。方法32只SD健康雄性大鼠,随机分为对照组、低剂量组、中剂量组和高剂量组,每组8只。纳米硫化铅混悬液灌胃染毒,染毒剂量分别为25、50、100mg/kg,对照组给予等体积的生理盐水,染毒30d。计算大鼠睾丸脏器系数,检测大鼠血清中雄激素水平,观察精子成活率、畸形率。结果各组大鼠睾丸脏器系数比较,差异均有统计学意义(P<0.05);染毒高剂量组最高,对照组最低。各组大鼠血清中睾酮含量比较,差异有统计学意义(P<0.05);高剂量组最高,对照组最低。染毒后大鼠精子存活率降低,高剂量组最低,对照组最高,差异有统计学意义(P<0.05);各组精子畸形率比较,差异无统计学意义(P>0.05)。结论纳米硫化铅对雄性大鼠精子存活率有一定影响,但对精子畸形率影响并不明显。
Objective To investigate the effect of nano lead sulfide on spermatogenesis in male rats. Methods Thirty-two SD healthy male rats were randomly divided into control group, low dose group, middle dose group and high dose group, with 8 rats in each group. Nano-lead sulfide suspension administered by intragastric administration, exposure doses were 25,50,100 mg / kg, the control group was given an equal volume of saline, exposure 30d. The testis organ coefficient was calculated and the level of serum androgen was measured. The sperm survival rate and malformation rate were observed. Results There were significant differences in the testis organ coefficient between the two groups (P <0.05). The high dose group was the highest and the lowest in the control group. The levels of testosterone in serum of the rats in each group were significantly different (P <0.05), the highest in the high-dose group and the lowest in the control group. The rat sperm survival rate was reduced after exposure to the highest dose group, the lowest in the control group, the difference was statistically significant (P <0.05); sperm deformity rate in each group, the difference was not statistically significant (P> 0.05). Conclusion Nano-PbS has certain influence on sperm survival rate in male rats, but it has no obvious effect on sperm deformity rate.