应用高效液相色谱法测定122名我国汉族健康志愿者一次口服苯妥英钠(100mg)后24h尿中4’-羟基苯妥英(4’-hydroxyphenytoin,HPPH)与苯妥英(phenytoin,PHT)的比值。研究结果表明,122名志愿者的苯妥英羟化代谢呈二态性分布,分界点值(log_(10)HPPH/PHT)为1.70,12名小于1.70为弱代谢者,其余均为强代谢者,弱代谢者组与强代谢者组HPPH尿药浓度有显著性差异,而PHT尿药浓度无差异。我国汉族人群苯妥英羟化代谢缺陷的频发率为9.8％。 The ratio of 4’-hydroxyphenytoin (HPPH) to phenytoin (PHT) in urine of 122 Chinese Han healthy volunteers after oral administration of phenytoin sodium (100mg) was determined by HPLC. The results showed that phenytoin in 122 volunteers showed a bimodal hydroxylation metabolism, the cut-off point value (log_ (10) HPPH / PHT) was 1.70, and the less than 1.70 were weak metabolizers. The others were strong metabolizers, There was a significant difference in urinary drug concentrations of HPPH between the poor metabolizer group and the strong metabolizer group, while there was no difference between the PHT urinary drug concentrations. Han Chinese population phenytoin hydroxylated metabolic frequency of frequent occurrence of 9.8%.