植物组织与器官培养必须在无菌条件下进行,除在接种前对培养基,接种环境和所用工具进行严格消毒外,对实验材料的消毒更为重要.由于试验材料是生活的植物组织,消毒时既要把材料上附着的微生物杀死又不能伤害组织或器官,而且消毒时所采用的药剂,浓度和处理时间往往因材料的具体情况而定,处理不当容易引起污染甚至使实验失败. 消毒前首先要选择适当的消毒剂.消毒剂 Plant tissue and organ culture must be performed under aseptic conditions, except for the strict disinfection of the culture medium, the inoculation environment and the tools used prior to inoculation, and disinfection of the test material is more important. Since the test material is the living plant tissue, disinfection It is necessary to kill the microorganisms attached to the material without injuring the tissues or organs, and the medicaments, concentration and processing time used in disinfection are often determined by the specific conditions of the materials, and improper handling can easily cause pollution or even make the experiment unsuccessful. First, select the appropriate disinfectant. Disinfectant