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目的：研究辅佐细胞在非肽类抗原ＭＥＰ激活γδＴ细胞中的作用及机制。方法：用花环沉降法分离出正常人外周血中的Ｔ细胞，通过细胞计数和ＦＡＣＳ，观察ＭＥＰ激活γδＴ细胞时辅佐细胞的作用。结果：ＭＥＰ激活γδＴ细胞需要有ＩＬ－２的持续存在。ＭＥＰ和ＩＬ－２联合作用于Ｔ细胞可少量激活其中的γδＴ细胞，但与ＩＬ－２的单独作用无明显差异。加入辅佐细胞能大大促进ＭＥＰ和ＩＬ－２联合激活γδＴ细胞的作用，且明显强于ＩＬ－２的单独作用。辅佐细胞分泌的上清对ＭＥＰ激活γδＴ细胞无明显促进作用。结论：ＭＥＰ激活γδＴ细胞需要辅佐细胞的参与，可能主要通过与Ｔ细胞间的直接接触而起作用。
Objective: To investigate the role and mechanism of adjuvant cells in the activation of γδT cells by non-peptide antigen MEP. Methods: Peripheral blood T cells were isolated from the normal human by means of rosette sedimentation. The effects of MEP on the activation of γδT cells were observed by cell counting and FACS. RESULTS: MEP activated γδT cells require the persistence of IL-2. The combined effect of MEP and IL-2 on T cells could activate γδT cells in a small amount, but there was no significant difference with IL-2 alone. Addition of adjuvant cells can greatly promote the joint activation of γδT cells by MEP and IL-2, and is significantly stronger than IL-2 alone. Adjuvant cells secreted supernatants MEP activated γδT cells had no significant role in promoting. Conclusion: The activation of γδT cells by MEP requires the participation of adjuvant cells and may play a role mainly through direct contact with T cells.