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目的：观察１甲基，４苯基１，２，３ ，６四氢吡啶（ ＭＰＴＰ） 和多巴胺对大鼠脑线粒体损伤作用。方法：大鼠脑线粒体与ＭＰＴＰ和多巴胺温孵后测定膜流动性、膜电位、线粒体肿胀、总ＡＴＰ酶、呼吸链复合酶活性及线粒体Ｈ２Ｏ２ ，Ｏ·２ 和丙二醛（ＭＤＡ）含量。结果：二者可降低线粒体膜流动性，ＭＰＴＰ损伤线粒体膜电位，对线粒体肿胀、总ＡＴＰ酶活性无影响；多巴胺不改变膜电位，却抑制总ＡＴＰ酶活性，导致线粒体肿胀。二者均增加线粒体中ＭＤＡ 含量。多巴胺使Ｈ２Ｏ２ 和Ｏ·２ 生成增加，ＭＰＴＰ仅增加Ｈ２Ｏ２ 生成。多巴胺抑制呼吸链复合酶活性，ＭＰＴＰ略有激活作用。结论：ＭＰＴＰ与多巴胺损伤线粒体作用的不同， 可能与通过不同途径刺激氧自由基生成有关。
Objective: To observe the effects of 1-methyl, 4-phenyl 1,2,3,4-tetrahydropyridine (MPTP) and dopamine on rat brain mitochondrial damage. Methods: Membrane fluidity, membrane potential, mitochondrial swelling, total ATPase, respiratory chain complex enzyme activities and mitochondrial H2O2, O2 and malondialdehyde (MDA) contents were measured after incubation of rat brain mitochondria with MPTP and dopamine. Results: Both of them reduced the fluidity of mitochondrial membrane, and MPTP could damage mitochondrial membrane potential and had no effect on mitochondrial swelling and total ATPase activity. Dopamine did not change membrane potential, but inhibited total ATPase activity, resulting in swelling of mitochondria. Both increase mitochondrial MDA content. Dopamine increases H2O2 and O.2 production and MPTP only increases H2O2 production. Dopamine inhibits respiratory chain complex enzyme activity, MPTP slightly activated. Conclusion: The difference of mitochondrial function between MPTP and dopamine may be related to the stimulation of oxygen free radical production through different ways.