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目的探讨小肠型克罗恩病(CD)肠黏膜中DLG1和DLG5的表达以及与临床关联分析。方法收集CD患者小肠手术切除标本45例,另收集15例小肠肿瘤以及右半结肠肿瘤手术标本中正常小肠组织作为对照组,采用免疫组织化学方法研究DLG1和DLG5在CD组患者和对照组小肠黏膜上皮组织中的表达,并进行临床观察分析。结果与对照组比较,CD患者小肠黏膜上皮细胞DLG1主要表达在细胞质中,两组患者DLG1表达部位有明显差别(P<0.01),表达量无明显差别。与对照组比较,CD组患者小肠黏膜上皮细胞DLG5均表达在细胞质中,CD组患者DLG5的表达量明显降低(P<0.01)。DLG1表达部位和DLG5表达量与CD患者临床特征无明显关联。结论 DLG5表达降低可能参与CD的发病过程。
Objective To investigate the expression of DLG1 and DLG5 in intestinal mucosa of small intestine type Crohn disease (CD) and its clinical correlation analysis. Methods Collected 45 cases of small intestine resected specimens from CD patients and collected 15 cases of small intestine tumors and normal small intestine tissues of right colon cancer as control group. Immunohistochemistry was used to detect the expression of DLG1 and DLG5 in CD group and control group Epithelial tissue expression, and clinical observation and analysis. Results Compared with the control group, the expression of DLG1 in small intestinal mucosal epithelial cells of CD patients was mainly expressed in the cytoplasm. The expression of DLG1 was significantly different between the two groups (P <0.01). There was no significant difference between the two groups. Compared with the control group, the DLG5 of small intestinal mucosal epithelial cells in CD group were all expressed in the cytoplasm, and the expression of DLG5 in CD group was significantly decreased (P <0.01). There was no significant correlation between the expression of DLG1 and the expression of DLG5 in clinical features of CD patients. Conclusion The decreased expression of DLG5 may be involved in the pathogenesis of CD.