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目的探索不同剂量慢性染锰对大鼠空间学习和记忆能力的影响。方法将40只SD大鼠随机分为对照组和低、中、高剂量组,每组10只,分别腹腔注射生理盐水和5、10和20 mg/kg的无菌Mn Cl2·4H2O溶液,5 d/周,连续18周。于染锰的第6、12、18周,采用Morris水迷宫试验检测大鼠的学习记忆能力。结果在第6、12、18周的定位航行试验中,中、高剂量组大鼠的总路程均大于对照组,差异有统计学意义(P<0.05);在第12和18周的空间探索试验中,中、高剂量组大鼠的平台象限时间及其百分比、平台象限路程及其百分比明显少于对照组,差异有统计学意义(P<0.05)。结论慢性染锰可损害大鼠的空间学习记忆能力。
Objective To explore the effect of different doses of chronic manganese on spatial learning and memory in rats. Methods Forty Sprague-Dawley rats were randomly divided into control group and low, medium and high dose groups, 10 rats in each group. Rats were injected intraperitoneally with normal saline and 5, 10 and 20 mg / kg sterile MnCl 2 · 4H 2 O. 5 d / week for 18 weeks. At the 6th, 12th and 18th weeks of manganese doping, Morris water maze test was used to detect the learning and memory ability of rats. Results At the 6th, 12th and 18th weeks of positioning navigation test, the total distance of rats in middle and high dose groups was longer than that in control group (P <0.05). At the 12th and 18th weeks of space exploration In the middle and high dose groups, the platform quadrant time and its percentage, the platform quadrant distance and its percentage were significantly less than those in the control group, with significant differences (P <0.05). Conclusion Chronic manganese exposure can impair spatial learning and memory in rats.