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采用脂质体转导法将人中性粒细胞防御素 HNP1 c DNA重组真核表达质粒 p Babe- Neo- HNP1 导入无血清培养的人气管粘膜上皮细胞 ;制备 HNP多克隆抗血清 ,用免疫组化法检测防御素 (Hum an neutrophil peptide- 1,HNP1 )在气管上皮细胞的表达。结果表明 :脂质体转导法可使 HNP1 c DNA重组真核表达质粒 p Babe- Neo- HNP1 在人气管粘膜上皮细胞有效表达 ,免疫组化显示转导组织呈强阳性反应。本实验为进一步研究应用内源性抗生素肽转基因治疗呼吸道感染性疾病打下了基础。
The human eukaryotic cell defensin HNP1 c DNA recombinant eukaryotic expression plasmid p Babe-Neo-HNP1 was introduced into human bronchial epithelial cells cultured in serum by liposome-mediated method. Polyclonal antiserum against HNP was prepared by immunization Method to detect the expression of Hum an neutrophil peptide-1 (HNP1) in tracheal epithelial cells. The results showed that the liposome transduction method could effectively express p Babe-Neo-HNP1, a HNP1c DNA recombinant plasmid, in human epithelial cells of the trachea. Immunohistochemistry showed that the transduced tissue was strongly positive. This experiment laid the foundation for further research on the application of endogenous antibiotic peptide transgene in the treatment of respiratory tract infectious diseases.