论文部分内容阅读
目的 系统研究 3种一氧化氮合酶 (nitricoxidesynthase ,NOS)在生殖周期中小鼠子宫分布变化 ,探讨内源性一氧化氮 (nitricoxide,NO)在生殖周期中可能的生理作用。 方法 免疫组织化学LSAB法 ,比色法。 结果 神经型一氧化氮合酶 (nNOS ,NOS1)在生殖周期小鼠子宫有较为稳定的表达 ,大量的阳性细胞主要分布在子宫内膜上皮 ,内膜基质 ,血管内皮 ,腺上皮及肌层 ;内皮型一氧化氮合酶 (eNOS ,NOS3)在妊娠中期小鼠子宫表达明显增强 ,蜕膜上皮 ,腺上皮和血管内皮呈强阳性标记 ;诱导型一氧化氮合酶 (iNOS ,NOS2 )在妊娠早期表达最强 ,妊娠中、晚期表达稍下降。阳性标记主要分布在肌层 ,血管内皮 ,腺上皮 ,内膜上皮及内膜基质 ;然而动情期小鼠子宫未见iNOS阳性标记。此外 ,还测定了生殖周期小鼠血清NO水平变化。 结论 3种NOS同工酶在生殖周期小鼠子宫可能既协作又分工 ,由其产生的内源性NO对雌鼠动情、胚胎植入、分娩前子宫静息状态的维持、分娩始动以及产后子宫修复等生理过程可能均有调节作用。
Objective To study the changes of the distribution of three kinds of nitric oxide synthase (NOS) during the reproductive cycle in mice and to explore the possible physiological role of endogenous nitric oxide (NO) in the reproductive cycle. Methods Immunohistochemical LSAB method, colorimetric method. Results NOS (NOS1) expressed more stable in reproductive cycle mouse uteri. A large number of positive cells were mainly distributed in endometrial stroma, endometrial stroma, vascular endothelium, glandular epithelium and myometrium. Endothelial nitric oxide synthase (eNOS, NOS3) in the second trimester of pregnancy significantly increased expression of the uterus, decidual epithelial cells, glandular epithelium and vascular endothelial was strongly positive markers; inducible nitric oxide synthase (iNOS, NOS2) in pregnancy Early expression of the strongest, during pregnancy, late expression decreased slightly. Positive markers were mainly located in the muscular layer, vascular endothelium, glandular epithelium, endometrial epithelium and intima stroma; however, iNOS-positive markers were not seen in estrous mouse uterus. In addition, changes in serum NO levels in reproductive cycle mice were also measured. Conclusions The three kinds of NOS isoenzymes may cooperate and divide labor in reproductive cycle mouse uterus. Endogenous nitric oxide (NO) produced by the three kinds of NOS isozymes may induce estrus, embryo implantation, maintenance of uterine resting state before delivery, onset of labor and postpartum Uterine repair and other physiological processes may have a regulatory role.