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幽门螺杆菌(Hp)存在于大多数人的胃中,能引起慢性胃炎、消化性溃疡,并有诱发胃癌的危险性.作者选用能自然感染Hp的非人灵长类动物恒河猴为模型,评估预防Hp的重组脲酶(rUre)菌苗的安全性和效力.实验动物为9月龄雌性恒河猴,实验前用ELISA(抗原为HP超声裂解物和脲酶)检测了109只动物的抗Hp IgG抗体,选出60只具最低ELISA滴度的动物随机分成免疫
Helicobacter pylori (Hp) is present in the stomach of most people and can cause chronic gastritis, peptic ulcer, and the risk of gastric cancer. The authors selected non-human primate rhesus monkeys that naturally infected with Hp as a model , To assess the safety and efficacy of a recombinant urease (rUre) vaccine against H. pylori The experimental animals were 9-month-old female rhesus monkeys and tested for anti-109 antibodies using ELISA (antigenic HP sonicated lysate and urease) before the experiment Hp IgG antibody, 60 animals with the lowest ELISA titer were selected and randomly divided into immunizations