论文部分内容阅读
目的:探讨不同睡眠剥夺时间对大鼠认知功能的影响以及对下丘脑内单胺类神经递质去甲肾上腺素、多巴胺、五羟吲哚乙酸、五羟色胺的含量的影响。方法:32只健康雄性wistar大鼠随机分为4组,即96 h、120 h、144 h睡眠剥夺,正常对照组。利用睡眠剥夺箱建立大鼠SD模型,避暗穿梭法测试大鼠认知功能,高效液相电化学检测法测定下丘脑内单胺类神经递质含量。结果:大鼠避暗穿梭实验,与对照组比较,96 h、120 h组大鼠潜伏期显著缩短(P<0.05);与对照组比较,各组大鼠下丘脑内NA含量均有下降(P<0.05);与对照组比较,各组大鼠下丘脑内DA含量均显著下降,(P<0.01),96 h、120 h、144 h组间比较,表现出含量逐渐减少的趋势;与对照组比较,各组5-HIAA含量均有上升,且120 h组明显高于其他各组(P<0.05),其他组无显著性差异(P>0.05);与对照组比较,各组5-HT含量均有升高,120 h、144 h组显著升高(P<0.01),96 h组无显著性(P>0.05)。结论:睡眠剥夺可以使大鼠中枢NA、DA含量下降,5-HIAA、5-HT含量升高,且随着睡眠剥夺时间的延长,变化更为明显,这可能是睡眠剥夺损害认知功能的原因之一。
Objective: To investigate the effect of different sleep deprivation time on cognitive function and the effect of monoamine neurotransmitters norepinephrine, dopamine, pentoxynoacetate and serotonin in the hypothalamus. Methods: Thirty-two healthy male Wistar rats were randomly divided into 4 groups: sleep deprivation at 96 h, 120 h, 144 h, and normal control group. The SD model was established by sleep deprivation box, the cognitive function of rats was tested by avoiding shuttle and the content of monoamine neurotransmitters was measured by HPLC. Results: Compared with the control group, the latency of rats in 96 h and 120 h groups was significantly shorter (P <0.05). Compared with the control group, the content of NA in the hypothalamus of rats decreased <0.05). Compared with the control group, the content of DA in hypothalamus of rats in each group was significantly decreased (P <0.01), and the contents of DA decreased gradually in 96 h, 120 h and 144 h groups The levels of 5-HIAA in each group increased, and the levels of 5-HIAA in 120 h group were significantly higher than those in other groups (P <0.05), but no significant difference was found in other groups (P> 0.05) HT content increased in 120 h, 144 h group was significantly increased (P <0.01), 96 h group was not significant (P> 0.05). Conclusion: Sleep deprivation can reduce the content of NA, DA and the content of 5-HIAA and 5-HT in the central nervous system of rats, and more obviously with the prolongation of sleep deprivation, which may be the cognitive impairment of sleep deprivation one of the reasons.