青黛是一种常用中药,有清热解毒、凉血定惊功效,其有效成分主要为靛蓝(Indigo)和靛玉红(Indirubin).关于青黛的质量控制,中国药典(1990年版)采用分光光度法测定靛蓝.目前,用等吸收点双波长法测定靛蓝和靛玉红的含量已有报道,但双波长法的量测点较少,其误差较大,灵敏度低.本文采用双波长量测标准加入分光光度法测 Indigo and indirubin are the main active ingredients of Qingdai, a commonly used traditional Chinese medicine, which has functions of detoxifying and cooling blood, and Chinese Pharmacopoeia (1990 edition) adopts the method of spectrophotometry Determination of indigo.At present, the determination of indigo and indirubin by the same absorption point double-wavelength method has been reported, but the dual-wavelength method has fewer measurement points, the error is larger and the sensitivity is lower.In this paper, Add spectrophotometry