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本研究分离纯化鸭肝HDL受体并对其理化性质作初步探讨.将北京鸭肝脏制成匀浆,再经超速离心以及去污剂β-吡喃葡萄糖苷(OG)处理等步骤制备肝细胞膜的可溶性组份.用Immuno-Blotting技术鉴定出其中有可与HDL或载脂蛋白AI(apo AI)结合的物质.采用制备性SDS-PAGE(浓度为10％和7％)以及回收电泳技术从可溶性膜组份中分离纯化HDL受体蛋白.该蛋白质用Immuno-Blotting技术验证只结合HDL或apo AI而不结合低密度脂蛋白(LDL)或apo E. 分析性SDS-PAGE电泳测得其分子量为80 000左右;等电聚焦电泳发现其有两个主要多态型(PI值
This study isolated and purified duck liver HDL receptor and its physical and chemical properties as a preliminary study of the Beijing duck liver homogenate, and then by ultracentrifugation and detergent β-glucopyranoside (OG) and other steps to prepare the liver cell membrane Of the soluble components were identified by Immuno-Blotting technology which can be combined with HDL or apolipoprotein AI (apo AI) using preparative SDS-PAGE (10% and 7% concentration) and the recovery of electrophoresis from HDL receptor protein was isolated and purified from the soluble membrane fractions using Immuno-Blotting technique to verify that it binds only HDL or apo AI without binding to low density lipoprotein (LDL) or apo E. The molecular weight of the HDL receptor is determined by analytical SDS-PAGE electrophoresis About 80 000; isoelectric focusing electrophoresis found that there are two major polymorphisms (PI value