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目的进一步了解鼠、螨、人间HFRSV的传播关系和抗原型别。方法将从陕西HFRS疫区鼠、螨、人中分离出的HFRSV进行病毒毒力测定，并用单克隆抗体（抗NP）和多聚酶链反应（RT—PCR）进行分型。结果从该疫区鼠、螨、人中所分离的HFRSV毒力测定、抗NP单克隆抗体滴度检测均显示有I型（Hantaan型，HTN型）和Ⅱ型（Seoul型，SEO型）双型反应特征。进一步用RT—PCR扩增鉴定，表明从鼠、螨、人中所分离的HFRSV—RNA均扩增出Ⅰ型和Ⅱ型所特异的460hP和650hP片段。结论该结果证明从鼠、螨、人中所分离的HFRSV其抗原性是一致的，并在鼠、螨、人中构成相互传播的关系；对维持HFRS疫源地有重要的作用。
Objective To further understand the transmission relationship and antigenic type of HFRSV in mice, mites and humans. Methods The HFRSV isolated from rats and mites in Shaanxi Province were assayed for virus virulence and classified by monoclonal antibody (NP) and polymerase chain reaction (RT-PCR). Results The virulence of HFRSV and the titer of anti-NP monoclonal antibody isolated from the infected mice, mites and human showed that both type I (type Hantaan, type HTN) and type II (type Seoul and type SEO) Type reaction characteristics. Further identification by RT-PCR indicated that both the 460hP and 650hP fragments specific for type I and type II were amplified from HFRSV-RNA isolated from mice, mites and humans. Conclusion The results demonstrate that the antigenicity of HFRSV isolated from mice, mites and humans is consistent and that it has a reciprocal transmission relationship in rats, mites and humans; it plays an important role in the maintenance of HFRS.