在实验室内,用东乡伊蚊和斑点按蚊叮吸一名来自菲律宾南吕宋索索贡省的志愿者的血。两周后将蚊虫冷冻,置玻璃板上,滴加数滴RPMI-1640,用玻管滚压辗碎。将碎物放在一带有细网的不锈钢漏斗内,用含青霉素、链霉素(各100μg/ml)的RPMI-1640冲洗过滤,收集班氏丝虫第三期幼虫(L_3)。将L_3置入盛有RPMI-1640、抗菌素、失 In the laboratory, blood was drawn from a volunteer from Sosung, Southern Luzon, Philippines, using the Aedes aloe and Anopheles stephensi. Two weeks later the mosquitoes were frozen and placed on a glass plate. A few drops of RPMI-1640 were added dropwise and the tube was rolled and crushed. The pieces were placed in a stainless steel funnel with a fine mesh and filtered through RPMI-1640 containing penicillin and streptomycin (100 μg / ml each) to collect the third larva of Bancroft’s filariasis (L_3). The L_3 into the filled with RPMI-1640, antibiotics, lost