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目的了解重症监护病房(ICU)甲氧西林耐药金黄色葡萄球菌(MRSA)的分子流行病学特点。方法采用随机引物聚合酶链反应(AP-PCR)法对2004年华中科技大学同济医学院附属同济医院ICU病房分离的25株MRSA作同源性分析。结果25株MRSA的AP-PCR图谱有7种类型(A~G型),主要型别为A型10株、B型6株、C型3株、D型3株及E、F、G型各1株。结论医院获得性MRSA是多重耐药菌,在ICU病房流行的MRSA菌株主要是A、B基因型。
Objective To investigate the molecular epidemiological characteristics of methicillin-resistant Staphylococcus aureus (MRSA) in intensive care unit (ICU). Methods 25 MRSA isolates isolated from ICU ward of Tongji Medical College affiliated to Tongji Medical College of Huazhong University of Science and Technology in 2004 were analyzed by random primer polymerase chain reaction (AP-PCR). Results The AP-PCR profiles of 25 strains of MRSA were classified into 7 types (A to G). The main types were A type 10 strains, B type 6 strains, C type 3 strains, D type 3 strains and E, F, G type Each one. Conclusion Hospital-acquired MRSA is a multi-drug resistant strain. The most common MRSA strains in the ICU ward are A and B genotypes.