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目的了解1～5岁肾小球微小病变(MCD)患儿肾小球基底膜(GBM)的厚度。方法选择年龄为1～5岁,无家族遗传病史或其他病史、不伴有血尿、经临床诊断为原发性肾病综合征,免疫荧光染色全部阴性或仅有少量非特异性沉积,光镜未见异常,并经透射电镜确诊为MCD的病例,在透射电镜下观察到至少2个肾小球的35个病例入选。10 000倍下,根据等距离曲线移动原则对每个样本的2个肾小球分别拍照10张,以4000 nm宽度设定测试网,从测试线与内皮细胞相交的点向GBM作垂直线与足细胞相交处的距离为GBM截面宽度,据此计算出GBM调和平均厚度。结果和结论随年龄增长GBM厚度增加,部分年龄段差别有统计学意义,尚需进一步研究。
Objective To investigate the thickness of glomerular basement membrane (GBM) in children with 1 ~ 5 years old glomerular minor lesion (MCD). Methods Ages 1-5 years old, no family history or other medical history, no hematuria, clinically diagnosed as primary nephrotic syndrome, immunofluorescence staining of all negative or only a small amount of non-specific deposition, no light microscope Abnormal, and confirmed by transmission electron microscopy MCD cases, observed in the transmission electron microscopy at least two cases of glomerular 35 cases were enrolled. 10 000 times, according to the principle of equidistance curve moving two glomeruli for each sample were taken 10, 4000 nm width test network set, from the intersection of the test line and endothelial cells to GBM for vertical lines and The distance between podocyte intersections is the width of GBM section, and the average thickness of GBM reconciliation is calculated accordingly. RESULTS AND CONCLUSIONS The thickness of GBM increased with age, and some differences in age groups were statistically significant and further study was needed.