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目的:通过研究不同月龄大鼠阴茎组织内细胞凋亡及CO含量变化,进一步探讨勃起功能障碍发生机制。方法:雄性Wistar大鼠24只分为3组(每组8只),成年组(8月龄)、老年组(16月龄)和衰老组(24月龄)。取阴茎组织用苏木精-伊红(HE)染色,原位末端转移酶标记法(TUNEL)检测细胞凋亡,改良双波长法检测CO含量。结果:增龄大鼠阴茎组织中细胞凋亡显著增多(成年组:4.38±1.06,老年组:9.38±1.69,衰老组:18.50±1.60),CO含量减少[成年组:(12.19±0.87)nmol/μg,老年组:(7.93±0.63)nmol/μg,衰老组:(5.68±0.62)nmol/μg],组间差异均有显著性(P<0.05)。CO含量与细胞凋亡的变化呈现良好的负相关性(r=-0.889,P<0.01)。结论:增龄大鼠阴茎组织中细胞凋亡与CO含量呈负相关,CO可能通过调控细胞凋亡机制从而参与了雄性性功能障碍的发生。
OBJECTIVE: To investigate the mechanism of erectile dysfunction by studying the changes of apoptosis and CO content in the penile tissue of rats of different months. Methods: Twenty-four male Wistar rats were divided into 3 groups (8 in each group), adult group (8 months old), aged group (16 months old) and aging group (24 months old). The tissue of the penis was stained with hematoxylin and eosin (HE), apoptosis was detected by TUNEL, and the CO content was detected by the improved dual-wavelength method. Results: Apoptosis was significantly increased in the penile tissues of aged rats (adult group: 4.38 ± 1.06, elderly group: 9.38 ± 1.69, aging group: 18.50 ± 1.60) and decreased CO content (12.19 ± 0.87) nmol / μg in aged group: (7.93 ± 0.63) nmol / μg in aged group and (5.68 ± 0.62) nmol / μg in aged group, respectively). There was significant difference between the two groups (P <0.05). CO content and apoptosis showed a good negative correlation (r = -0.889, P <0.01). CONCLUSION: Apoptosis in penile tissue of aged rats is negatively correlated with CO content. CO may be involved in the development of male sexual dysfunction through regulating apoptosis mechanism.