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目的研究发现石胆酰牛磺酸及其他一些胆汁酸是M3受体的部分激动剂,而胆汁反流到胃和食管又是一个普遍现象。本研究旨在探索胃、食管黏膜的M3受体表达情况。方法分别使用羊和兔抗人M3受体的多克隆IgG抗体A和B,采用免疫组化,在11例人胃和食管的不同部位黏膜对M3受体进行定位。结果使用抗体A发现胃泌酸腺表面黏液细胞、小凹细胞、颈部细胞和壁细胞,及食管鳞状上皮的棘层细胞表现为细胞质染色,使用抗体B发现除上述细胞的胞质染色外,人胃壁肌层平滑肌细胞表现为胞膜和胞质共同染色。结论研究揭示M3受体表达于人胃泌酸腺表面黏液细胞、小凹细胞、腺颈部细胞、壁细胞和食管鳞状上皮的棘层细胞。
OBJECTIVE: It has been found that bile cholic acid and some other bile acids are partial agonists of M3 receptors, and that bile reflux to the stomach and esophagus is a common phenomenon. This study aimed to explore the gastric and esophageal mucosa M3 receptor expression. Methods The polyclonal IgG antibodies A and B of goat and rabbit anti-human M3 receptor were used respectively, and immunohistochemistry was used to locate the M3 receptor in mucosa of 11 human gastric and esophageal mucosa. Results The results showed that gastric mucosa, parietal cells, parietal cells and parietal cells of gastric acid-gland surface and stratum spinosi cells of esophageal squamous epithelium were found to be cytoplasm stained by antibody A. Antibody B was found to be cytosolic stained Human gastric parietal smooth muscle cells showed common membrane and cytoplasmic staining. Conclusions The study revealed that the M3 receptor is expressed on the stratum spinosum of mucosa, pits, glandular neck cells, parietal cells, and esophageal squamous epithelium in human gastric acid secretion.