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目的探讨芦荟苷对PC12细胞氧糖剥夺再灌注损伤是否具有保护作用。方法将PC12细胞随机分为正常组、模型组、尼莫地平组、芦荟苷低、中、高剂量组,采用氧糖剥夺再灌注损伤模型,通过采用2-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)检测细胞存活率,紫外分光光度计检测细胞乳酸脱氢酶(LDH)漏出率,荧光分光光度计测定细胞内活性氧(ROS)含量;运用激光共聚焦显微镜(CLSM)测定PC12细胞线粒体膜电位水平(MMP);通过生化方法测定丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性。结果与正常组相比,芦荟苷(10,20,和40μg/ml)可以显著升高细胞存活率,降低细胞内乳酸脱氢酶漏出率、活性氧含量和线粒体膜电位水平,并且降低丙二醛和升高超氧化物歧化酶活性。结论芦荟苷对PC12细胞氧糖剥夺再灌注损伤具有明显的保护作用。
Objective To investigate whether aloin can protect PC12 cells against oxygen-glucose deprivation-reperfusion injury. Methods PC12 cells were randomly divided into normal group, model group, nimodipine group, low, middle and high dose of aloin glycosides. The models were induced by oxygen sugar deprivation and reperfusion injury. By using 2- (4,5-dimethylthiazol-2- yl) -2,5-diphenyltetrazolium bromide (MTT) was used to detect the cell viability. The leakage rate of lactate dehydrogenase (LDH) was measured by ultraviolet spectrophotometer. The content of reactive oxygen species (ROS) in cells was measured by fluorescence spectrophotometer. The level of mitochondrial membrane potential (MMP) in PC12 cells was determined by confocal microscopy (CLSM). The content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) were measured by biochemical methods. Results Compared with the normal group, aloe glycosides (10, 20, and 40 μg / ml) significantly increased cell viability, decreased intracellular leakage of lactate dehydrogenase, reactive oxygen species and mitochondrial membrane potential, Aldehyde and increase superoxide dismutase activity. Conclusion Aloin has a protective effect on oxygen-glucose deprivation-reperfusion injury in PC12 cells.