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目的 探讨特发性肺纤维化 (IPF)患者肺泡巨噬细胞蛋白激酶C(PKC)活性的变化。方法 应用放射活性测定法检测 9例健康对照者和 15例临床诊断为IPF的患者经纤维支气管镜检查获得的支气管肺泡灌洗液 (BALF)中肺泡巨噬细胞的PKC活性。结果 IPF患者BALF肺泡巨噬细胞总PKC活性 (57 86± 8 6 0pmol·min 1·mg 1vs 4 6 0 2± 11 73pmol·min 1·mg 1)、胞浆 (89 83± 2 1 94pmol·min 1·mg 1vs 6 5 73± 2 7 91pmol·min 1·mg 1)和胞膜PKC活性 (39 6 7± 8 98pmol·min 1·mg 1vs 32 77± 4 96pmol·min 1·mg 1)均明显高于对照组 (P <0 0 1、P <0 0 5和P <0 0 5) ,AM的总PKC活性与BALF中细胞总数呈正相关 (r=0 8135,P <0 0 1) ,胞膜PKC活性也与之呈正相关 (r=0 5917,P <0 0 5)。结论 PKC作为细胞活化的信号传导通路与肺间质病的发生发展密切相关
Objective To investigate the changes of protein kinase C (PKC) activity in alveolar macrophages in patients with idiopathic pulmonary fibrosis (IPF). Methods The activity of PKC in alveolar macrophages in bronchoalveolar lavage fluid (BALF) obtained by fiberoptic bronchoscopy was detected by radioactivity assay in 9 healthy controls and 15 clinically diagnosed IPF patients. Results The total PKC activity of BALF alveolar macrophages in IPF patients was 57 86 ± 80 6 pmol·L -1 · mg 1 vs 4 6 0 2 ± 11 73 pmol·L -1 · mg -1 and 89 83 ± 2 1 94 pmol·min 1 · mg 1 vs 6 5 73 ± 2 7 91 pmol·min 1 · mg 1) and membrane PKC activity (39 6 7 ± 8 98 pmol·min 1 · mg 1 vs 32 77 ± 4 96 pmol·min 1 · mg 1) Compared with the control group (P <0.01, P <0.05 and P <0.05), the total PKC activity in AM was positively correlated with the total number of cells in BALF (r = 0 8135, P 0 01) There was also a positive correlation between membrane PKC activity (r = 0 5917, P <0 05). Conclusions PKC as a signal pathway of cell activation is closely related to the occurrence and development of interstitial lung disease