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目的探讨发育期大鼠暴露低剂量双酚A(BPA)对免疫器官及调节性T细胞功能的影响及机制。方法初断乳20只雄性大鼠按照体重随机分为2组,分别为对照组(含1%无水乙醇的水)和双酚A染毒组(1μg/ml),每组10只,通过饮水方式进行BPA染毒8周。实验结束后,计算大鼠胸腺系数和脾脏系数;ELISA方法检测大鼠血清肿瘤坏死因子-α(TNF-α)、干扰素γ(IFN-γ)和白细胞介素-4(IL-4)的含量;流式细胞术检测大鼠脾脏CD4+T细胞中调节性T细胞(Treg)数量和脾细胞线粒体膜电位。结果与对照组比较,BPA组(1μg/ml)大鼠胸腺系数和脾脏系数未见明显差异;血清TNF-α和IFN-γ水平明显升高(P<0.01和P<0.05),IL-4水平没有明显差异;脾脏细胞中Treg数量降低(P<0.05),脾细胞线粒体膜电位下降(P<0.05)。结论低剂量BPA(1μg/ml)暴露对发育期雄鼠的免疫器官脏器系数未见显著影响,但可影响Th1免疫应答;其机制可能与BPA增加机体炎症反应和诱导脾细胞凋亡有关。
Objective To investigate the effect of low dose bisphenol A (BPA) on the function of immune organs and regulatory T cells in developing rats. Methods Twenty weaned infant rats were randomly divided into two groups according to body weight: control group (water containing 1% ethanol) and BPA (1μg / ml) Drinking water for BPA exposure for 8 weeks. The thymus coefficient and spleen coefficient of rats were calculated after the experiment, and the levels of tumor necrosis factor-α (TNF-α), interferon γ (IFN-γ) and interleukin-4 . The number of Tregs and the mitochondrial membrane potential of splenocytes in splenic CD4 + T cells were detected by flow cytometry. Results Compared with the control group, there was no significant difference in the thymus coefficient and spleen coefficient between the BPA group (1μg / ml) and the serum levels of TNF-α and IFN-γ (P <0.01 and P <0.05) The level of Treg in spleen cells was decreased (P <0.05), and the mitochondrial membrane potential in spleen cells was decreased (P <0.05). Conclusions Exposure of low dose BPA (1μg / ml) to immune organs in developing mice has no significant effect on organ coefficient, but may affect Th1 immune response; its mechanism may be related to BPA increase the body inflammatory response and induce apoptosis of spleen cells.