论文部分内容阅读
目的研究Kazal基序逆向诱导富含半胱氨酸蛋白(RECK)在支气管哮喘小鼠肺组织的表达及地塞米松处理后对其表达的影响。方法 BALB/c小鼠分为哮喘组、对照组、地塞米松组,每组10只。采用鸡卵清蛋白(OVA)腹腔注射致敏及雾化吸入的方法制作哮喘模型,进行支气管肺泡灌洗液(BALF)中嗜酸性粒细胞(EOS)及淋巴细胞(Lym)分类计数;HE染色观察气道炎症细胞浸润及气道重塑情况,实时荧光定量PCR检测RECK mRNA的表达,免疫组织化学法检测RECK蛋白的表达及定位。结果与对照组和地塞米松组相比,哮喘组BALF中细胞总数、EOS明显增多;RECK mRNA在哮喘组表达较对照组、地塞米松组明显降低。免疫组织化学结果显示RECK主要表达于气道上皮细胞、上皮下炎症细胞。哮喘组表达最低,对照组表达最高,地塞米松组表达较哮喘组有所增高。结论 RECK在哮喘小鼠肺组织中表达明显降低,而地塞米松可上调RECK的表达。
Objective To investigate the effect of Kazal motif reversely inducing RECK expression in lung tissue of asthmatic mice and the effect of dexamethasone on its expression. Methods BALB / c mice were divided into asthma group, control group and dexamethasone group, with 10 rats in each group. Asthma model was made by intraperitoneal injection of chicken ovalbumin (OVA) and inhalation by atomization, and the count of eosinophils (EOS) and lymphocytes (Lym) in bronchoalveolar lavage fluid (BALF) The infiltration of airway inflammatory cells and airway remodeling were observed. The expression of RECK mRNA was detected by real-time fluorescence quantitative PCR. The expression and location of RECK protein were detected by immunohistochemistry. Results Compared with control group and dexamethasone group, the total number of cells and EOS in BALF of asthmatic group were significantly increased. The expression of RECK mRNA in asthmatic group was significantly lower than that of control group and dexamethasone group. Immunohistochemistry showed that RECK was mainly expressed in airway epithelial cells and subepithelial inflammatory cells. Asthma group had the lowest expression, while the control group had the highest expression. Dexamethasone group had higher expression than the asthma group. Conclusion The expression of RECK in lung tissue of asthmatic mice was significantly decreased, while dexamethasone up-regulated the expression of RECK.