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溶藻弧菌(Vibrio alginolyticus)、哈维氏弧菌(Vibrio harveyi)、副溶血弧菌(Vibrio parahaemolytic-us)是引起大黄鱼(Pseudosciaena crocea)溃疡病的3种主要致病菌.将试验大黄鱼随机分成4组,分别腹腔注射哈维氏弧菌、溶藻弧菌、副溶血弧菌和灭菌生理盐水,在感染后的不同时间采样,通过白细胞数量、白细胞分类计数、NBT阳性细胞数量以及溶菌酶活力的变化趋势来探讨3种致病弧菌感染对大黄鱼非特异性免疫功能的影响.结果表明,大黄鱼各项血液指标的变化主要发生在感染后的1～7 d.在人工感染的初期,各实验组大黄鱼外周血的白细胞数量、NBT阳性细胞数量呈现先增加后降低的趋势,淋巴细胞、嗜中性粒细胞百分比显著降低(p<0.05),单核细胞百分比显著提高(p<0.05);随着时间的延长,白细胞数量开始下降;淋巴细胞、嗜中性粒细胞百分比有所上升,7 d以后各类血细胞数量变化不显著.其中哈氏弧菌感染组在白细胞数量、NBT阳性细胞数量等多个指标上的变化最为明显.血清中溶菌酶活性显著高于脾组织,鱼体受感染后两者溶菌酶活性均较对照组有显著提高,不同致病菌与大黄鱼非特异性免疫功能之间呈现一定的时效差异.研究认为反映鱼体受感染后从应激反应发生至非特异性免疫功能的发挥主要发生在病原菌感染前期,不同致病菌与大黄鱼非特异性免疫功能之间呈现一定的时效差异.
Vibrio alginolyticus, Vibrio harveyi and Vibrio parahaemolyticus are the three major pathogenic bacteria that cause ulcer disease in large yellow croaker (Pseudosciaena crocea) The yellow croaker was randomly divided into 4 groups. The animals were intraperitoneally injected with Vibrio harveyi, Vibrio alginolyticus, Vibrio parahaemolyticus and sterilized normal saline, and sampled at different times after infection. The numbers of white blood cells, white blood cell count, NBT positive cells And lysozyme activity to investigate the effects of three kinds of pathogenic Vibrio infections on the non-specific immune function of large yellow croaker.The results showed that the changes of blood indexes of large yellow croaker occurred mainly from 1 to 7 days after infection In the early stage of infection, the number of leukocytes and the number of NBT positive cells in the experimental group increased firstly and then decreased, the percentage of lymphocytes and neutrophils was significantly decreased (p <0.05), and the percentage of mononuclear cells was significantly increased (p <0.05). With the extension of time, the number of white blood cells began to decline, the percentage of lymphocytes and neutrophils increased, and the number of blood cells changed insignificantly after 7 days Vibrio harveyi group in the number of white blood cells, the number of NBT positive cells and other indicators of the most obvious changes in serum lysozyme activity was significantly higher than the spleen, the two infected fish lysozyme activity than the control group Significantly increased, different pathogenic bacteria and non-specific immune function of large yellow croaker showed some time differences between studies reflect that the reaction of fish from stress to non-specific immune function occurs mainly in the pre-infection of pathogens, different Pathogenic bacteria and non-specific immune function of large yellow croaker showed some differences between the aging.