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目的观察在大鼠诱发性肝癌中肝脏组织中细胞角质素19(CK19)及C-kit(CD117)蛋白的表达,探讨其在肝癌发生发展过程中的意义。方法利用二乙基亚硝胺(DEN)制备诱发性大鼠肝癌模型,在此模型的基础上,HE染色观察肝组织的形态学变化,免疫组织化学法检测CK19及C-kit蛋白的表达。结果根据肝组织形态学变化将诱癌过程分为肝细胞损伤期、肝细胞增生-硬化期和肝细胞癌变期。胆管上皮、卵圆细胞、癌周组织中的细胞和少量癌细胞上均可见CK19蛋白阳性表达;C-kit蛋白表达在卵圆细胞,并随着诱癌的进行出现向肝小叶内穿插的现象。结论 CK19及C-kit蛋白标记的卵圆细胞是大鼠DEN诱发性肝癌发生发展的启动细胞。
Objective To observe the expression of CK19 and C-kit (CD117) in hepatic tissue of rats with induced hepatocellular carcinoma (HCC) and to explore its significance in the development and progression of HCC. Methods The induced hepatocellular carcinoma model was induced by diethylnitrosamine (DEN). On the basis of this model, the morphological changes of liver were observed by HE staining. The expression of CK19 and C-kit protein were detected by immunohistochemistry. Results According to the morphological changes of liver tissue, the process of carcinogenesis was divided into hepatocellular injury, hepatocellular hyperplasia-cirrhosis and hepatocellular carcinoma. The positive expression of CK19 protein was found on the bile duct epithelium, oval cells, peritumoral tissues and small amount of cancer cells. The expression of C-kit protein was in oval cells and intercalated into the hepatic lobules with carcinogenesis . Conclusion CK19 and C-kit protein-labeled oval cells are the initiating cells for the development of DEN-induced hepatocellular carcinoma in rats.